Development, Performance Evaluation and Clinical Application of A Rapid SARS-CoV-2 IgM and IgG Test Kit Based on Automated Fluorescence Immunoassay
The continued COVID-19 epidemic has made a big impact on well being, economies, and societies everywhere in the world. Though RT-PCR-based nucleic acid detection has been primarily used within the analysis of COVID-19, it’s time-consuming with restricted software situations and should be operated by certified personnel. Antibody take a look at, significantly point-of-care antibody testing, is an acceptable complement to nucleic acid take a look at because it offers fast, transportable, and cost-effective detection of infections.
On this research, a fast antibody take a look at equipment was developed based mostly on fluorescence immunochromatography for the delicate, correct, and automatic detection of IgM and IgG antibodies towards SARS-CoV-2 in human serum, plasma, and complete blood samples inside 10 minutes. The sensitivity, specificity, precision, and stability of the take a look at equipment had been of excellent efficiency.
No cross-activity and no interference was noticed. Within the multiple-center parallel research, 223 samples from hospitalized sufferers had been used to judge the scientific specificity of the take a look at. Each SARS-CoV-2 IgM and IgG achieved a scientific specificity of 98.21%. The scientific sensitivities of SARS-CoV-2 IgM and IgG had been 79.54% and 87.45%, respectively, amongst 733 RT-PCR confirmed SARS-Cov-2 samples. For the mixed IgM and IgG assays, the sensitivity and specificity had been 89.22% and 96.86%, respectively. Our outcomes exhibit that the mixed use of IgM and IgG may function a extra appropriate various detection technique for COVID-19 sufferers, and the developed equipment is of nice public well being significance for the prevention and management of the COVID-19 pandemic.
Comparability of the SARS-CoV-2 Fast Antigen Check to the Actual Star Sars-CoV-2 RT PCR Package
There’s an ongoing want for dependable antigen assays for well timed and simple detection of people with acute SARS-CoV-2 an infection. Utilizing 75 swabs from sufferers beforehand examined constructive by SARS-CoV-2 PCR and 75 swabs from sufferers beforehand examined damaging by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the SARS-CoV-2 Fast Antigen Check (Roche).
We decided a specificity of 96 %. The assay’s sensitivity with samples with a cycle threshold of < 25, 25 – <30, 30 – <35, and > = 35 was 100%, 95%, 44.8% and 22.2%, respectively. We conclude that sensitivity and specificity of the antigen assay is inferior to the PCR assay. Nevertheless, the antigen assay could also be a fast and simple to carry out various for differentiation of people contagious for SARS-CoV-2 from non-contagious people.
Efficacy of a industrial take a look at equipment to find out early being pregnant in cows utilizing complete blood and blood serum
The target of this research was to find out the efficacy of the Fassisi®BoviPreg visible take a look at equipment (Fassisi®BoviPreg) in figuring out early pregnancies in cows by measuring the pregnancy-associated glycoprotein (PAG) in complete blood and blood serum. The research was performed on 50 cows, the synthetic insemination (AI) dates of which had been designated as day 0.
Being pregnant analysis was carried out with transrectal ultrasonography (USG), and serum samples had been concurrently collected and used with Fassisi®BoviPreg to find out pregnancies on 30 days after AI. The outcomes of the Fassisi®BoviPreg take a look at on serum and complete blood samples, respectively, on 30 days after AI had been as follows: sensitivity, 61.54% and 50.0%; specificity, 79.17% and 75%; accuracy, 70.0% and 62.0%; constructive predictive values, 76.2% and 68.4%; damaging predictive values, 65.5% and 58.1%; false-positive diagnoses, 23.8% and 31.6%; and false-negative diagnoses, 34.5% and 41.9%.
On day 50 after AI, sensitivities had been 63.64% and 50.0%, specificities had been 100.0% and 100.0%, and accuracies had been 75.0% and 65.62% in serum and complete blood, respectively. Larger being pregnant charges had been obtained utilizing Fassisi®BoviPreg in cows with ≥ 5 ng/mL P4 (P < 0.001). In conclusion, the outcomes from the Fassisi®BoviPreg checks on cows on 30 and 50 days after AI confirmed that using blood serum is extra correct and appropriate than that of complete blood. The outcomes additionally confirmed a better confidence stage in specificity 50 days after AI.
Optimum Allocation of COVID-19 Check Kits Amongst Accredited Checking Facilities within the Philippines
Testing is essential for early detection, isolation, and remedy of coronavirus illness (COVID-19)-infected people. Nevertheless, in resource-constrained international locations such because the Philippines, take a look at kits have restricted availability. As of 11 April 2020, there are 11 testing facilities within the nation which were accredited by the Division of Well being (DOH) to conduct testing.
On this paper, we use nonlinear programming (NLP) to find out the optimum proportion allocation of COVID-19 take a look at kits amongst accredited testing facilities within the Philippines that offers an equitable likelihood to all contaminated people to be examined. Heterogeneity in testing accessibility, inhabitants density of municipalities, and the capability of testing services are included within the mannequin.
Our outcomes present that the vary of optimum allocation per testing heart are as follows: Analysis Institute for Tropical Drugs (4.17-6.34%), San Lazaro Hospital (14.65-24.03%), College of the Philippines-Nationwide Institutes of Well being (16.25-44.80%), Lung Heart of the Philippines (15.8-26.40%), Baguio Normal Hospital Medical Heart (0.58-0.76%), The Medical Metropolis, Pasig Metropolis (5.96-25.51%), St. Luke’s Medical Heart, Quezon Metropolis (1.09-6.70%), Bicol Public Well being Laboratory (0.06-0.08%), Western Visayas Medical Heart (0.71-4.52%), Vicente Sotto Memorial Medical Heart (1.02-2.61%), and Southern Philippines Medical Heart (≈ 0.01%).
FAM-Spacer-Phe-CMK FLISP™ Assay Kit |
KF17322 |
Neuromics |
25 Tests |
EUR 349.2 |
FAM-Spacer-Phe-CMK FLISP™ Assay Kit |
KF17323 |
Neuromics |
100 Tests |
EUR 834 |
FAM-Phe-DAP Serine Protease Assay Kit |
abx299013-25tests |
Abbexa |
25 tests |
EUR 698.4 |
|
SR-101-Leu-CMK FLISP™ Assay Kit |
KF17321 |
Neuromics |
100 Tests |
EUR 834 |
FAM-Leu-CMK Serine Protease Assay Kit |
abx299011-25tests |
Abbexa |
25 tests |
EUR 679.2 |
|
OPTIONAL GREEN FILTER (572NM) |
GDBL-GREEN |
CORNING |
1/pk |
EUR 1262.4 |
Description: Lab Equipment; Axygen Branded EQ |
SR-101-Phe-CMK FLISP™ Assay Kit |
KF17318 |
Neuromics |
25 Tests |
EUR 349.2 |
SR-101-Phe-CMK FLISP™ Assay Kit |
KF17319 |
Neuromics |
100 Tests |
EUR 834 |
Polycaspase Assay Kit, green |
KF17200 |
Neuromics |
25 Tests |
EUR 364.8 |
FAM-Phe-CMK Serine Protease Assay Kit |
abx299010-25tests |
Abbexa |
25 tests |
EUR 679.2 |
|
Malachite Green Phosphate Assay Kit |
abx298878-100Assays |
Abbexa |
100 Assays |
EUR 453.6 |
|
Caspase 1 Assay Kit, green |
KF17201 |
Neuromics |
25 Tests |
EUR 390 |
Caspase 2 Assay Kit, green |
KF17202 |
Neuromics |
25 Tests |
EUR 364.8 |
Caspase 6 Assay Kit, green |
KF17204 |
Neuromics |
25 Tests |
EUR 364.8 |
Caspase 8 Assay Kit, green |
KF17205 |
Neuromics |
25 Tests |
EUR 364.8 |
Caspase 9 Assay Kit, green |
KF17206 |
Neuromics |
25 Tests |
EUR 364.8 |
Caspase 10 Assay Kit, green |
KF17207 |
Neuromics |
25 Tests |
EUR 364.8 |
Caspase 13 Assay Kit, green |
KF17208 |
Neuromics |
25 Tests |
EUR 364.8 |
Malachite Green Phosphate Assay Kit |
POMG-25H |
BioAssay Systems |
2500 |
EUR 330 |
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C. |
Malachite Green Phosphate Assay Kit |
Z5030012 |
Biochain |
2,500 assays |
EUR 410.4 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Malachite Green Phosphate Assay kit |
65-POMG-25H |
Gentaur Genprice |
2500 tests |
EUR 333 |
HDAC Fluorogenic Assay Kit (Green) |
50034 |
BPS Bioscience |
96 rxns. |
EUR 450 |
Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening_x000D_and profiling applications. The kit comes in a convenient 96-well format, with all the_x000D_reagents necessary for 100 fluorescent HDAC activity measurements. In_x000D_addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor,_x000D_Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC_x000D_Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_steps on a microtiter plate are needed to analyze the HDAC activity level. First,_x000D_the HDAC fluorometric substrate, containing an acetylated lysine side chain, is_x000D_incubated with purified HDAC enzyme. The deacetylation sensitizes the_x000D_substrate so subsequent treatment with the Lysine Developer produces a_x000D_fluorophore that can then be measured using a fluorescence reader at 485 nm_x000D_(excitation)/528 nm (emission)._x000D__x000D_Our other HDAC kit (#50033) contains a substrate that is excited at wavelengths 350-380 nm and fluoresces at wavelengths 440-460 nm. However, the substrate in this kit fluoresces at longer (green) wavelengths: 485 nm(excitation)/528 nm (emission). It is most useful when the sample or inhibitor fluoresces at wavelengths that overlap those of our other HDAC substrate |
FAM-cAMP PDE IV substrate *Green Fluorescence* |
13602 |
AAT Bioquest |
0.5 umol |
EUR 367.2 |
|
FAM-cGMP PDE V substrate *Green Fluorescence* |
13604 |
AAT Bioquest |
0.5 umol |
EUR 367.2 |
|
Morpheus HT-96 Green Screen |
M-MD1-47-GREEN |
MiTeGen |
96 x 1 ml ml |
EUR 280 |
|
Description: Morpheus HT-96 Green Screen |
Caspase 3 & 7 Assay Kit, green |
KF17203 |
Neuromics |
25 Tests |
EUR 364.8 |
EZCell? Phagocytosis Assay Kit (Green Zymosan) |
K397-100 |
Biovision |
each |
EUR 516 |
Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 |
M-1895.0001 |
Bachem |
1.0mg |
EUR 385.2 |
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net |
Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 |
M-1895.0005 |
Bachem |
5.0mg |
EUR 1430.4 |
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net |
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence) |
STA-342 |
Cell Biolabs |
96 assays |
EUR 762 |
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence) |
STA-342-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3058.8 |
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence) |
STA-349 |
Cell Biolabs |
192 assays |
EUR 644.4 |
Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner. |
EZCell? Phagocytosis Assay Kit (Green E. coli) |
K963-100 |
Biovision |
each |
EUR 516 |
Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 |
M-2350.0001 |
Bachem |
1.0mg |
EUR 441.6 |
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net |
Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 |
M-2350.0005 |
Bachem |
5.0mg |
EUR 1662 |
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net |
Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2 |
M-2670.0001 |
Bachem |
1.0mg |
EUR 400.8 |
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net |
Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2 |
M-2670.0005 |
Bachem |
5.0mg |
EUR 1488 |
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net |
Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 |
H-2638.0001 |
Bachem |
1.0mg |
EUR 283.2 |
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net |
Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 |
H-2638.0005 |
Bachem |
5.0mg |
EUR 1023.6 |
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net |
Cell Meter™ Autophagy Assay Kit *Green Fluorescence* |
23002 |
AAT Bioquest |
200 Tests |
EUR 367.2 |
|
Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence* |
11401 |
AAT Bioquest |
200 Tests |
EUR 262.8 |
|
Amplite™ Fluorimetric Glutathione Assay Kit *Green Fluorescence* |
10055 |
AAT Bioquest |
200 Tests |
EUR 262.8 |
|
Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2 |
M-2225.0001 |
Bachem |
1.0mg |
EUR 445.2 |
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net |
Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2 |
M-2225.0005 |
Bachem |
5.0mg |
EUR 1585.2 |
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net |
5-FAM |
20-abx095073 |
Abbexa |
-
EUR 627.60
-
EUR 444.00
-
EUR 1528.80
|
|
|
FAM-cAMP |
60200 |
BPS Bioscience |
100 µl |
EUR 245 |
Description: The fluorogenic PDE substrate FAM-cAMP is used for cAMP-dependent phosphodiesterase assays. It has been used in activity assays for PDE1, PDE2, PDE3, PDE4, PDE7, PDE8, PDE10 and PDE11. |
FAM-cGMP |
60201 |
BPS Bioscience |
100 µl |
EUR 235 |
Description: The fluorogenic PDE substrate FAM-cGMP is used for cGMP-dependent phosphodiesterase assays. It has been used in activity assays for PDE5, PDE6 and PDE9. |
N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 |
M-2145.0001 |
Bachem |
1.0mg |
EUR 529.2 |
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net |
N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 |
M-2145.0005 |
Bachem |
5.0mg |
EUR 2010 |
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net |
Cell Meter™ Cell Viability Assay Kit *Green Fluorescence* |
22786 |
AAT Bioquest |
500 Tests |
EUR 262.8 |
|
Cell Meter™ TUNEL Apoptosis Assay Kit *Green Fluorescence* |
22849 |
AAT Bioquest |
50 Tests |
EUR 367.2 |
|
Amplite™ MMP-3 Activity Assay Kit *Green Fluorescence* |
13512 |
AAT Bioquest |
100 Tests |
EUR 367.2 |
|
Amplite™ Fluorimetric HDAC Activity Assay Kit *Green Fluorescence* |
13601 |
AAT Bioquest |
200 Tests |
EUR 262.8 |
|
Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence* |
12601 |
AAT Bioquest |
500 Tests |
EUR 367.2 |
|
Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence* |
11953 |
AAT Bioquest |
500 Tests |
EUR 262.8 |
|
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size |
STA-342-T |
Cell Biolabs |
20 assays |
EUR 414 |
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence) |
STA-347 |
Cell Biolabs |
96 assays |
EUR 734.4 |
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF. |
OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence) |
STA-347-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2913.6 |
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF. |
EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence |
K452-100 |
Biovision |
each |
EUR 588 |
EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence |
K453-100 |
Biovision |
each |
EUR 588 |
EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence |
K497-100 |
Biovision |
each |
EUR 588 |
Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2 |
M-2520.0001 |
Bachem |
1.0mg |
EUR 385.2 |
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net |
Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2 |
M-2520.0005 |
Bachem |
5.0mg |
EUR 1430.4 |
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net |
Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence* |
5524 |
AAT Bioquest |
200 Tests |
EUR 262.8 |
|
Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence* |
22730 |
AAT Bioquest |
200 Tests |
EUR 262.8 |
|
Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence* |
13456 |
AAT Bioquest |
100 tests |
EUR 367.2 |
|
Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence* |
13500 |
AAT Bioquest |
500 Tests |
EUR 262.8 |
|
Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence* |
13503 |
AAT Bioquest |
500 Tests |
EUR 367.2 |
|
Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence* |
13510 |
AAT Bioquest |
100 Tests |
EUR 367.2 |
|
Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence* |
16315 |
AAT Bioquest |
100 Tests |
EUR 420 |
|
Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence* |
23005 |
AAT Bioquest |
100 Tests |
EUR 334 |
EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence |
K459-100 |
Biovision |
each |
EUR 660 |
DAP Antibody |
1-CSB-PA170450 |
Cusabio |
|
|
|
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100 |
DAP Antibody |
1-CSB-PA262326 |
Cusabio |
|
|
|
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200 |
DAP Antibody |
1-CSB-PA01034A0Rb |
Cusabio |
|
|
|
Description: A polyclonal antibody against DAP. Recognizes DAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:50-1:200 |
DAP Antibody |
1-CSB-PA007640 |
Cusabio |
|
|
|
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000 |
DAP Antibody |
1-CSB-PA006496GA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA |
DAP Antibody |
7389-002mg |
ProSci |
0.02 mg |
EUR 206.18 |
|
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2). |
DAP Antibody |
7389-01mg |
ProSci |
0.1 mg |
EUR 523.7 |
|
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2). |
DAP Peptide |
7389P |
ProSci |
0.05 mg |
EUR 197.7 |
Description: (CT) DAP Peptide |
DAP antibody |
70R-32429 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit polyclonal DAP antibody |
DAP Antibody |
43505-100ul |
SAB |
100ul |
EUR 302.4 |
DAP antibody |
70R-16735 |
Fitzgerald |
50 ul |
EUR 522 |
Description: Rabbit polyclonal DAP antibody |
DAP antibody |
38736-100ul |
SAB |
100ul |
EUR 302.4 |
DAP Antibody |
35710-100ul |
SAB |
100ul |
EUR 302.4 |
6 FAM SE |
A8771-10 |
ApexBio |
10 mg |
EUR 208.8 |
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein. |
6 FAM SE |
A8771-100 |
ApexBio |
100 mg |
EUR 877.2 |
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein. |
6 FAM SE |
A8771-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 238.8 |
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein. |
6 FAM SE |
A8771-50 |
ApexBio |
50 mg |
EUR 543.6 |
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein. |
5-FAM-Woodtide |
H-7644.0001 |
Bachem |
1.0mg |
EUR 472.8 |
Description: Sum Formula: C89H133N21O26S; CAS# [1566528-51-5] net |
Amplite™ Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence* |
5528 |
AAT Bioquest |
200 Tests |
EUR 262.8 |
|
Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence* |
22798 |
AAT Bioquest |
200 Tests |
EUR 262.8 |
|
Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence* |
22799 |
AAT Bioquest |
200 Tests |
EUR 367.2 |
|
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence* |
11503 |
AAT Bioquest |
200 Tests |
EUR 315.6 |
|
Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence* |
16060 |
AAT Bioquest |
200 Tests |
EUR 367.2 |
|
Amplite™ Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* |
10056 |
AAT Bioquest |
200 Tests |
EUR 367.2 |
|
OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size |
STA-347-T |
Cell Biolabs |
20 assays |
EUR 400.8 |
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF. |
EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence) |
K560-100 |
Biovision |
each |
EUR 588 |
EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence) |
K592-100 |
Biovision |
each |
EUR 588 |
EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence) |
K714-100 |
Biovision |
each |
EUR 614.4 |
Islr ELISA Kit| Mouse Immunoglobulin superfamily containing leu |
EF015231 |
Lifescience Market |
96 Tests |
EUR 826.8 |
Frit Kit |
FRIT-KIT |
Next Advance |
1each |
EUR 148.8 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit |
K2085-100 |
ApexBio |
100 assays |
EUR 634.8 |
Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit |
K564-100 |
Biovision |
each |
EUR 646.8 |
FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11268) |
OKSA11268 |
Aviva Systems Biology |
25 Tests |
EUR 709.2 |
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11269) |
OKSA11269 |
Aviva Systems Biology |
100 Tests |
EUR 1828.8 |
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICACASPASE-3/7 KIT (OKSA11270) |
OKSA11270 |
Aviva Systems Biology |
25 Tests |
EUR 709.2 |
Description: Description of target: Caspase FLICA™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA™ reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe. The Caspase FLICA™ Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates. ;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11272) |
OKSA11272 |
Aviva Systems Biology |
25 Tests |
EUR 717.6 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11273) |
OKSA11273 |
Aviva Systems Biology |
100 Tests |
EUR 1860 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICA CASPASE-1 KIT (OKSA11274) |
OKSA11274 |
Aviva Systems Biology |
25 Tests |
EUR 709.2 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-1 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICA CASPASE-1 KIT (OKSA11275) |
OKSA11275 |
Aviva Systems Biology |
100 Tests |
EUR 1827.6 |
Description: Description of target: The FAM FLICATM Capase 1 kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, FAM, and FMK to make a quick and flexible method to analyze active capases in apoptotic cells. These kits measure apoptosis by detecting active caspases in whole, living cells. They don't work by using antibodies or an ELISA. Instead, their methodology is based a unique cell-permeable and non-cytotoxic reagent called Fluorochrome Inhibitor of Caspases. These kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect, and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11276) |
OKSA11276 |
Aviva Systems Biology |
25 Tests |
EUR 709.2 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11277) |
OKSA11277 |
Aviva Systems Biology |
100 Tests |
EUR 1828.8 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11278) |
OKSA11278 |
Aviva Systems Biology |
25 Tests |
EUR 709.2 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11279) |
OKSA11279 |
Aviva Systems Biology |
100 Tests |
EUR 1828.8 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICAâ„¢ CASPASE-2 KIT (OKSA11282) |
OKSA11282 |
Aviva Systems Biology |
25 Tests |
EUR 668.4 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICA CASPASE-2 KIT (OKSA11283) |
OKSA11283 |
Aviva Systems Biology |
100 Tests |
EUR 1593.6 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
FAM FLICA CASPASE-10 KIT (OKSA11285) |
OKSA11285 |
Aviva Systems Biology |
100 Tests |
EUR 1875.6 |
Description: Description of target: FAM FLICAâ„¢ CASPASE-10 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
Column Packing Kit |
PACK-KIT |
Next Advance |
1pack |
EUR 1242 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2 |
M-2510.0001 |
Bachem |
1.0mg |
EUR 849.6 |
Description: Sum Formula: C66H82N16O17S; CAS# [1926163-82-7] net |
Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2 |
M-2510.0005 |
Bachem |
5.0mg |
EUR 3286.8 |
Description: Sum Formula: C66H82N16O17S; CAS# [1926163-82-7] net |
DAP Blocking Peptide |
DF7628-BP |
Affbiotech |
1mg |
EUR 234 |
Z-Dap-OH |
C-3315.0005 |
Bachem |
5.0g |
EUR 283.2 |
Description: Sum Formula: C11H14N2O4; CAS# [35761-26-3] |
Z-Dap-OH |
C-3315.0025 |
Bachem |
25.0g |
EUR 1009.2 |
Description: Sum Formula: C11H14N2O4; CAS# [35761-26-3] |
DAP Conjugated Antibody |
C35710 |
SAB |
100ul |
EUR 476.4 |
DAP cloning plasmid |
CSB-CL006496HU-10ug |
Cusabio |
10ug |
EUR 279.6 |
|
Description: A cloning plasmid for the DAP gene. |
DAP-3 Antibody |
20-abx141935 |
Abbexa |
-
EUR 444.00
-
EUR 727.20
-
EUR 360.00
|
|
|
DAP Rabbit pAb |
A6150-100ul |
Abclonal |
100 ul |
EUR 369.6 |
DAP Rabbit pAb |
A6150-200ul |
Abclonal |
200 ul |
EUR 550.8 |
DAP Rabbit pAb |
A6150-20ul |
Abclonal |
20 ul |
EUR 219.6 |
DAP Rabbit pAb |
A6150-50ul |
Abclonal |
50 ul |
EUR 267.6 |
Boc-Dap-OH |
A-3220.0001 |
Bachem |
1.0g |
EUR 151.2 |
Description: Sum Formula: C8H16N2O4; CAS# [73259-81-1] |
Boc-Dap-OH |
A-3220.0005 |
Bachem |
5.0g |
EUR 501.6 |
Description: Sum Formula: C8H16N2O4; CAS# [73259-81-1] |
Boc-Dap-OH |
A-3220.0025 |
Bachem |
25.0g |
EUR 1893.6 |
Description: Sum Formula: C8H16N2O4; CAS# [73259-81-1] |
Boc-Dap-OtBu |
A-4715.0001 |
Bachem |
1.0g |
EUR 370.8 |
Description: Sum Formula: C12H24N2O4; CAS# [77215-54-4] net |
Boc-Dap-OtBu |
A-4715.0005 |
Bachem |
5.0g |
EUR 1371.6 |
Description: Sum Formula: C12H24N2O4; CAS# [77215-54-4] net |
Polyclonal DAP Antibody |
APR06825G |
Leading Biology |
0.1 mg |
EUR 790.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human DAP . This antibody is tested and proven to work in the following applications: |
Fmoc-Dap-OH |
B-2385.0001 |
Bachem |
1.0g |
EUR 181.2 |
Description: Sum Formula: C18H18N2O4; CAS# [181954-34-7] |
Fmoc-Dap-OH |
B-2385.0005 |
Bachem |
5.0g |
EUR 618 |
Description: Sum Formula: C18H18N2O4; CAS# [181954-34-7] |
Fmoc-Dap-OH |
B-2385.0025 |
Bachem |
25.0g |
EUR 2358 |
Description: Sum Formula: C18H18N2O4; CAS# [181954-34-7] |
Anti-DAP antibody |
STJ27903 |
St John's Laboratory |
100 µl |
EUR 332.4 |
Description: This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
DAP Polyclonal Antibody |
A71011-050 |
EpiGentek |
50 ul |
EUR 302.5 |
DAP Polyclonal Antibody |
A71011-100 |
EpiGentek |
100 ul |
EUR 423.5 |
DAP Polyclonal Antibody |
A52804-020 |
EpiGentek |
20 ul |
EUR 117.7 |
DAP Polyclonal Antibody |
A52804-050 |
EpiGentek |
50 ul |
EUR 302.5 |
DAP Polyclonal Antibody |
A52804-100 |
EpiGentek |
100 ul |
EUR 423.5 |
DAP Polyclonal Antibody |
A52804 |
EpiGentek |
-
EUR 684.66
-
EUR 117.70
-
EUR 302.50
-
EUR 423.50
|
- 100 µg
- 20 ul
- 50 ul
- 100 ul
|
FAM azide, 5- isomer |
A8117-25 |
ApexBio |
25 mg |
EUR 470.4 |
Description: FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel. |
FAM azide, 5- isomer |
A8117-5 |
ApexBio |
5 mg |
EUR 199.2 |
Description: FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel. |
FAM azide, 6- isomer |
A8118-25 |
ApexBio |
25 mg |
EUR 470.4 |
Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer. |
FAM azide, 6- isomer |
A8118-5 |
ApexBio |
5 mg |
EUR 199.2 |
Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer. |
Aminooxy-5(6)-FAM |
90103 |
Biotium |
5MG |
EUR 290.4 |
Description: Minimum order quantity: 1 unit of 5MG |
Polyclonal USP9X / FAM Antibody |
APR13928G |
Leading Biology |
0.05mg |
EUR 580.8 |
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human USP9X / FAM . This antibody is tested and proven to work in the following applications: |
Our outcomes can function a information to the authorities in distributing the COVID-19 take a look at kits. These can be used for proposing further testing facilities and using the accessible take a look at kits correctly and equitably, which helps in “flattening” the epidemic curve.
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