Development, Performance Evaluation and Clinical Application of A Rapid SARS-CoV-2 IgM and IgG Test Kit Based on Automated Fluorescence Immunoassay

Development, Performance Evaluation and Clinical Application of A Rapid SARS-CoV-2 IgM and IgG Test Kit Based on Automated Fluorescence Immunoassay

Development, Performance Evaluation and Clinical Application of A Rapid SARS-CoV-2 IgM and IgG Test Kit Based on Automated Fluorescence Immunoassay

The continued COVID-19 epidemic has made a big impact on well being, economies, and societies everywhere in the world. Though RT-PCR-based nucleic acid detection has been primarily used within the analysis of COVID-19, it’s time-consuming with restricted software situations and should be operated by certified personnel. Antibody take a look at, significantly point-of-care antibody testing, is an acceptable complement to nucleic acid take a look at because it offers fast, transportable, and cost-effective detection of infections.

On this research, a fast antibody take a look at equipment was developed based mostly on fluorescence immunochromatography for the delicate, correct, and automatic detection of IgM and IgG antibodies towards SARS-CoV-2 in human serum, plasma, and complete blood samples inside 10 minutes. The sensitivity, specificity, precision, and stability of the take a look at equipment had been of excellent efficiency.

No cross-activity and no interference was noticed. Within the multiple-center parallel research, 223 samples from hospitalized sufferers had been used to judge the scientific specificity of the take a look at. Each SARS-CoV-2 IgM and IgG achieved a scientific specificity of 98.21%. The scientific sensitivities of SARS-CoV-2 IgM and IgG had been 79.54% and 87.45%, respectively, amongst 733 RT-PCR confirmed SARS-Cov-2 samples. For the mixed IgM and IgG assays, the sensitivity and specificity had been 89.22% and 96.86%, respectively. Our outcomes exhibit that the mixed use of IgM and IgG may function a extra appropriate various detection technique for COVID-19 sufferers, and the developed equipment is of nice public well being significance for the prevention and management of the COVID-19 pandemic.

Comparability of the SARS-CoV-2 Fast Antigen Check to the Actual Star Sars-CoV-2 RT PCR Package

There’s an ongoing want for dependable antigen assays for well timed and simple detection of people with acute SARS-CoV-2 an infection. Utilizing 75 swabs from sufferers beforehand examined constructive by SARS-CoV-2 PCR and 75 swabs from sufferers beforehand examined damaging by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the SARS-CoV-2 Fast Antigen Check (Roche).
We decided a specificity of 96 %. The assay’s sensitivity with samples with a cycle threshold of < 25, 25 – <30, 30 – <35, and > = 35 was 100%, 95%, 44.8% and 22.2%, respectively. We conclude that sensitivity and specificity of the antigen assay is inferior to the PCR assay. Nevertheless, the antigen assay could also be a fast and simple to carry out various for differentiation of people contagious for SARS-CoV-2 from non-contagious people.

Efficacy of a industrial take a look at equipment to find out early being pregnant in cows utilizing complete blood and blood serum

The target of this research was to find out the efficacy of the Fassisi®BoviPreg visible take a look at equipment (Fassisi®BoviPreg) in figuring out early pregnancies in cows by measuring the pregnancy-associated glycoprotein (PAG) in complete blood and blood serum. The research was performed on 50 cows, the synthetic insemination (AI) dates of which had been designated as day 0.
Being pregnant analysis was carried out with transrectal ultrasonography (USG), and serum samples had been concurrently collected and used with Fassisi®BoviPreg to find out pregnancies on 30 days after AI. The outcomes of the Fassisi®BoviPreg take a look at on serum and complete blood samples, respectively, on 30 days after AI had been as follows: sensitivity, 61.54% and 50.0%; specificity, 79.17% and 75%; accuracy, 70.0% and 62.0%; constructive predictive values, 76.2% and 68.4%; damaging predictive values, 65.5% and 58.1%; false-positive diagnoses, 23.8% and 31.6%; and false-negative diagnoses, 34.5% and 41.9%.
On day 50 after AI, sensitivities had been 63.64% and 50.0%, specificities had been 100.0% and 100.0%, and accuracies had been 75.0% and 65.62% in serum and complete blood, respectively. Larger being pregnant charges had been obtained utilizing Fassisi®BoviPreg in cows with ≥ 5 ng/mL P4 (P < 0.001). In conclusion, the outcomes from the Fassisi®BoviPreg checks on cows on 30 and 50 days after AI confirmed that using blood serum is extra correct and appropriate than that of complete blood. The outcomes additionally confirmed a better confidence stage in specificity 50 days after AI.
Development, Performance Evaluation and Clinical Application of A Rapid SARS-CoV-2 IgM and IgG Test Kit Based on Automated Fluorescence Immunoassay

Optimum Allocation of COVID-19 Check Kits Amongst Accredited Checking Facilities within the Philippines

Testing is essential for early detection, isolation, and remedy of coronavirus illness (COVID-19)-infected people. Nevertheless, in resource-constrained international locations such because the Philippines, take a look at kits have restricted availability. As of 11 April 2020, there are 11 testing facilities within the nation which were accredited by the Division of Well being (DOH) to conduct testing.
On this paper, we use nonlinear programming (NLP) to find out the optimum proportion allocation of COVID-19 take a look at kits amongst accredited testing facilities within the Philippines that offers an equitable likelihood to all contaminated people to be examined. Heterogeneity in testing accessibility, inhabitants density of municipalities, and the capability of testing services are included within the mannequin.
Our outcomes present that the vary of optimum allocation per testing heart are as follows: Analysis Institute for Tropical Drugs (4.17-6.34%), San Lazaro Hospital (14.65-24.03%), College of the Philippines-Nationwide Institutes of Well being (16.25-44.80%), Lung Heart of the Philippines (15.8-26.40%), Baguio Normal Hospital Medical Heart (0.58-0.76%), The Medical Metropolis, Pasig Metropolis (5.96-25.51%), St. Luke’s Medical Heart, Quezon Metropolis (1.09-6.70%), Bicol Public Well being Laboratory (0.06-0.08%), Western Visayas Medical Heart (0.71-4.52%), Vicente Sotto Memorial Medical Heart (1.02-2.61%), and Southern Philippines Medical Heart (≈ 0.01%).

FAM-Spacer-Phe-CMK FLISP™ Assay Kit
KF17322 25 Tests
EUR 291

FAM-Spacer-Phe-CMK FLISP™ Assay Kit
KF17323 100 Tests
EUR 695

FAM-Phe-DAP Serine Protease Assay Kit
abx299013-25tests 25 tests
EUR 582

SR-101-Leu-CMK FLISP™ Assay Kit
KF17321 100 Tests
EUR 695

FAM-Leu-CMK Serine Protease Assay Kit
abx299011-25tests 25 tests
EUR 566

OPTIONAL GREEN FILTER (572NM)
GDBL-GREEN 1/pk
EUR 1052
Description: Lab Equipment; Axygen Branded EQ

Polycaspase Assay Kit, green
KF17200 25 Tests
EUR 304

SR-101-Phe-CMK FLISP™ Assay Kit
KF17318 25 Tests
EUR 291

SR-101-Phe-CMK FLISP™ Assay Kit
KF17319 100 Tests
EUR 695

Caspase 1 Assay Kit, green
KF17201 25 Tests
EUR 325

Caspase 2 Assay Kit, green
KF17202 25 Tests
EUR 304

Caspase 6 Assay Kit, green
KF17204 25 Tests
EUR 304

Caspase 8 Assay Kit, green
KF17205 25 Tests
EUR 304

Caspase 9 Assay Kit, green
KF17206 25 Tests
EUR 304

Caspase 10 Assay Kit, green
KF17207 25 Tests
EUR 304

Caspase 13 Assay Kit, green
KF17208 25 Tests
EUR 304

Malachite Green Phosphate Assay Kit
abx298878-100Assays 100 Assays
EUR 378

Malachite Green Phosphate Assay Kit
POMG-25H 2500
EUR 275
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

Malachite Green Phosphate Assay Kit
Z5030012 2,500 assays
EUR 342
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Caspase 3 & 7 Assay Kit, green
KF17203 25 Tests
EUR 304

EZCell? Phagocytosis Assay Kit (Green Zymosan)
K397-100
EUR 430

FAM-Phe-CMK Serine Protease Assay Kit
abx299010-25tests 25 tests
EUR 566

FAM-cAMP PDE IV substrate *Green Fluorescence*
13602 0.5 umol
EUR 306

FAM-cGMP PDE V substrate *Green Fluorescence*
13604 0.5 umol
EUR 306

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)
STA-342 96 assays
EUR 635
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)
STA-342-5 5 x 96 assays
EUR 2549
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence)
STA-349 192 assays
EUR 537
Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

EZCell? Phagocytosis Assay Kit (Green E. coli)
K963-100
EUR 430

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
M-1895.0001 1.0mg
EUR 321
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
M-1895.0005 5.0mg
EUR 1192
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*
11401 200 Tests
EUR 219

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
M-2350.0001 1.0mg
EUR 368
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
M-2350.0005 5.0mg
EUR 1385
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net

Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2
M-2670.0001 1.0mg
EUR 334
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net

Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2
M-2670.0005 5.0mg
EUR 1240
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net

Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
H-2638.0001 1.0mg
EUR 236
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net

Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
H-2638.0005 5.0mg
EUR 853
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net

Dap/ Rat Dap ELISA Kit
ELI-31052r 96 Tests
EUR 886

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*
11953 500 Tests
EUR 219

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*
12601 500 Tests
EUR 306

Amplite™ MMP-3 Activity Assay Kit *Green Fluorescence*
13512 100 Tests
EUR 306

Amplite™ Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*
13601 200 Tests
EUR 219

Cell Meter™ Cell Viability Assay Kit *Green Fluorescence*
22786 500 Tests
EUR 219

Cell Meter™ TUNEL Apoptosis Assay Kit *Green Fluorescence*
22849 50 Tests
EUR 306

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size
STA-342-T 20 assays
EUR 345
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)
STA-347 96 assays
EUR 612
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)
STA-347-5 5 x 96 assays
EUR 2428
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K452-100
EUR 490

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K453-100
EUR 490

EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K497-100
EUR 490

Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2
M-2225.0001 1.0mg
EUR 371
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net

Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2
M-2225.0005 5.0mg
EUR 1321
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net

N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
M-2145.0001 1.0mg
EUR 441
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net

N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
M-2145.0005 5.0mg
EUR 1675
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net

Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
13456 100 tests
EUR 306

Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*
13500 500 Tests
EUR 219

Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence*
13503 500 Tests
EUR 306

Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*
13510 100 Tests
EUR 306

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*
16315 100 Tests
EUR 350

Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*
22730 200 Tests
EUR 219

Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
5524 200 Tests
EUR 219

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence
K459-100
EUR 550

Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2
M-2520.0001 1.0mg
EUR 321
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net

Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2
M-2520.0005 5.0mg
EUR 1192
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*
11503 200 Tests
EUR 263

Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*
16060 200 Tests
EUR 306

Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*
22798 200 Tests
EUR 219

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*
22799 200 Tests
EUR 306

Amplite™ Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
5528 200 Tests
EUR 219

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size
STA-347-T 20 assays
EUR 334
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K560-100
EUR 490

EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K592-100
EUR 490

EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K714-100
EUR 512

6-FAM
HY-66021 500mg
EUR 312

5-FAM
HY-66022 10mM/1mL
EUR 126

5-FAM
20-abx095073
  • EUR 523.00
  • EUR 370.00
  • EUR 1274.00
  • 1 g
  • 500 mg
  • 5 g

DAP Antibody
1-CSB-PA262326
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200

DAP Antibody
1-CSB-PA01034A0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DAP. Recognizes DAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:50-1:200

DAP Antibody
1-CSB-PA006496GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA

DAP Antibody
1-CSB-PA007640
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000

DAP Antibody
1-CSB-PA170450
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

DAP Antibody
35710-100ul 100ul
EUR 252

DAP siRNA
20-abx913570
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DAP siRNA
20-abx913571
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DAP siRNA
20-abx901410
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DAP antibody
38736-100ul 100ul
EUR 252

DAP Antibody
43505-100ul 100ul
EUR 252

DAP Antibody
ABD7628 100 ug
EUR 438

DAP Antibody
DF7628 200ul
EUR 304
Description: DAP Antibody detects endogenous levels of total DAP.

DAP antibody
70R-16735 50 ul
EUR 435
Description: Rabbit polyclonal DAP antibody

DAP Peptide
7389P 0.05 mg
EUR 164.75
Description: (CT) DAP Peptide

DAP antibody
70R-32429 100 ug
EUR 327
Description: Rabbit polyclonal DAP antibody

DAP Antibody
7389-002mg 0.02 mg
EUR 171.82
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).

DAP Antibody
7389-01mg 0.1 mg
EUR 436.42
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).
Our outcomes can function a information to the authorities in distributing the COVID-19 take a look at kits. These can be used for proposing further testing facilities and using the accessible take a look at kits correctly and equitably, which helps in “flattening” the epidemic curve.
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