Evaluation of a commercial dengue combo rapid test kit for the detection of NS1 and IgM

Evaluation of a commercial dengue combo rapid test kit for the detection of NS1 and IgM

Evaluation of a commercial dengue combo rapid test kit for the detection of NS1 and IgM

Correct and well timed prognosis is essential for dengue affected person administration as a consequence of no particular remedy is obtainable for the illness. The usage of speedy diagnostic checks (RDTs) might help in illness screening due to their simplicity and inexpensiveness however nonetheless, the efficiency of those checks must be rigorously evaluated. Right here, we report the efficiency of RVR Dengue Combo NS1-IgG/IgM Speedy Check for detection of dengue NS1 antigen and dengue-specific IgM utilizing 98 samples that had been screened initially utilizing Panbio Dengue IgM Seize ELISA and Panbio Dengue Early Speedy Check.

The optimistic % settlement (PPA) between RVR Dengue Combo NS1-IgG/IgM Speedy Check and the reference comparator checks was 77.8% and adverse % settlement (NPA) was 95.7% for NS1 (κ=0.748, P<0.001). As for IgM, the PPA and NPA was 54.5% and 100.0% respectively (κ=0.561, P<0.001). Combining each NS1 and IgM outcomes utilizing a logical OR operator gave the very best compromised PPA and NPA of 75.0% and 97.2% respectively (κ=0.743, P<0.001). In one other facet, all dengue adverse samples with excessive titer of rheumatoid issue (RF) and Hepatitis B floor Antigen (HBsAg) had been examined adverse for NS1, IgG and IgM utilizing this RDT thus indicating no cross-reactivity to those interference components.

Sensitivity and Specificity of Lateral Circulation Antigen Check Kits for COVID-19 in Asymptomatic Inhabitants of Quarantine Centre of Province 3

Background Practically after 6 months of the unfold of Corona Virus Illness 19, together with the world Nepal continues to be making an attempt to manage the unfold and stop normal inhabitants from buying it. With restricted assets in manpower, expertise and proof it has been a tough battle.
However with time and extra understanding of the virus new expertise to detect the virus are developing. It’s a main breakthrough within the diagnostic discipline as this helps us in not solely detecting the virus but additionally helps us to mobilize our human assets. This is available in a time the place the circumstances are rising at an alarming charge.
Though numbers of Polymerase Chain Response testing have elevated however because of the time consuming and the price clever, we want a quicker and equally dependable various. Antigen check permitted by totally different international locations can be utilized for level of care, screening and surveillance relying upon the necessities after calculating its sensitivity, specificity and accuracy.
Goal To search out out sensitivity and specificity of the Antigen check package for COVID-19. Methodology Antigen checks had been in contrast with Reverse Transcription Polymerase Chain Response as a reference customary in calculated pattern dimension of 113 topics in a excessive threat inhabitants. Each Reverse Transcription Polymerase Chain Response and antigen check had been carried out in a identical topic with in most of two days’ interval. Comfort sampling method was used to pick out the themes. Moral approval was taken from Nepal Well being Analysis Council earlier than information assortment.
Examine was completed from August to September 2020 from Quarantine heart of Province 3. Consequence There have been complete of 113 check carried out, amongst these 47 had been optimistic and 66 had been adverse in Reverse Transcription Polymerase Chain Response. After getting ready two by two desk, Sensitivity and specificity of the examined was calculated which got here out to be 85% and 100% respectively, with accuracy of 93.80%.
Conclusion Despite the fact that the sensitivity and specificity got here to be increased, this check needs to be interpreted cautiously relying upon the prevalence of Corona Virus Illness 19 in that exact group and the medical and epidemiological context of the one who has been examined. When doubtful by medical correlation needs to be confirmed with Reverse Transcription Polymerase Chain Response.

Pooling of Nasopharyngeal (NP) Swab Samples to Overcome International Scarcity of rRT-PCR COVID-19 Check Kits

The worldwide outbreak and speedy unfold of SARS-CoV-2 created an pressing want for big scale testing of populations. There’s a demand for top throughput testing protocols that can be utilized for environment friendly and speedy testing of medical specimens. We evaluated a pooled-PCR protocol for testing nasopharyngeal swabs utilizing identified optimistic/adverse and untested medical samples that had been assigned to swimming pools of 5 or 10. Six-hundred and thirty (630) samples had been used on this examine.
Evaluation of a commercial dengue combo rapid test kit for the detection of NS1 and IgM
Particular person optimistic samples with Ct values as excessive as 33 might be persistently detected when pooled with four adverse samples (pool of 5) and particular person optimistic samples with Ct values as much as 31 might be persistently detected when pooled with 9 adverse samples (pool of 10). Pooling of as much as 5 samples may be employed in laboratories for the prognosis of COVID-19 for environment friendly utilization of assets, speedy screening of a larger variety of individuals, and quicker reporting of check outcomes.

Challenges of Detecting Lead in Ingesting Water Utilizing at-Residence Check Kits

Lead in ingesting water stays a major human well being threat. At-home lead in water check kits might present customers with a handy and inexpensive choice to judge this threat, however their accuracy and reliability is unsure. This examine examined the flexibility of at-home lead check kits to detect various concentrations of dissolved and particulate lead in ingesting water.
Sixteen manufacturers representing 4 check package varieties (binary colour, binary strip, colorimetric vial, and colour strip) had been recognized. Most kits (12 of 16 manufacturers) weren’t appropriate for ingesting water evaluation, with lead detection limits of 5-20 mg/L. Binary strips detected dissolved lead at ingesting water-relevant ranges however did not detect particulate lead.

ELISA GENERAL ASSAY DILUENT

MBS238291-5x100mL 5x100mL
EUR 770

ELISA GENERAL ASSAY DILUENT

MBS239226-500mL 500mL
EUR 535

ELISA GENERAL ASSAY DILUENT

MBS239226-5x500mL 5x500mL
EUR 2240

ELISA GENERAL ASSAY DILUENT

MBS239227-1L 1L
EUR 680

ELISA GENERAL ASSAY DILUENT

MBS239227-5x1L 5x1L
EUR 2930

2x General Assay Diluent AD1

MBS258416-1L 1L
EUR 620

2x General Assay Diluent AD1

MBS258416-5x1L 5x1L
EUR 2660

General Serum Diluent

647 100 mL
EUR 57.25

General Serum Diluent

648 500 mL
EUR 227.5

General Serum Diluent

649 1 L
EUR 295

General Serum Diluent

MBS258402-100mL 100mL
EUR 160

General Serum Diluent

MBS258402-1L 1L
EUR 350

General Serum Diluent

MBS258402-500mL 500mL
EUR 295

General Serum Diluent

MBS258402-5x1L 5x1L
EUR 1450

2x General Serum Diluent, SD1

MBS258415-1L 1L
EUR 620

2x General Serum Diluent, SD1

MBS258415-5x1L 5x1L
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General Purpose Sample Diluent-SD1

KF17352 500 ml
EUR 349.2

Assay Diluent, 6ML

X017-6ML 6ML
EUR 73

IGM ASSAY DILUENT

MBS319902-25mL 25mL
EUR 370

IGM ASSAY DILUENT

MBS319902-5x25mL 5x25mL
EUR 1500

Assay Diluent, 12ML

X017-12ML 12ML
EUR 91

Assay Diluent Buffer

DS0231-100ML 1 unit
EUR 3.26
Description: Assay Diluent Buffer

Assay Diluent Buffer

DS0231-500ML 1 unit
EUR 15.45
Description: Assay Diluent Buffer

HISPEC ASSAY DILUENT

MBS238308-50mL 50mL
EUR 250

HISPEC ASSAY DILUENT

MBS238308-5x50mL 5x50mL
EUR 960

HISPEC ASSAY DILUENT

MBS238309-125mL 125mL
EUR 430

HISPEC ASSAY DILUENT

MBS238309-5x125mL 5x125mL
EUR 1760

HISPEC ASSAY DILUENT

MBS238310-500mL 500mL
EUR 1345

HISPEC ASSAY DILUENT

MBS238310-5x500mL 5x500mL
EUR 5870

Neptune Assay Diluent

MBS258388-100mL 100mL
EUR 160

Neptune Assay Diluent

MBS258388-1L 1L
EUR 355

Neptune Assay Diluent

MBS258388-500mL 500mL
EUR 305

Neptune Assay Diluent

MBS258388-5x1L 5x1L
EUR 1485

Neptune™ Assay Diluent

626 100 mL
EUR 57.25

Neptune™ Assay Diluent

627 500 mL
EUR 239

Neptune™ Assay Diluent

628 1 L
EUR 306

ANTIGEN DOWN ASSAY DILUENT

MBS238389-1L 1L
EUR 635

ANTIGEN DOWN ASSAY DILUENT

MBS238389-5x1L 5x1L
EUR 2730

Neptune Assay Diluent-AD3

KF17349 500 ml
EUR 292.8

ELISA NEPTUNE ASSAY DILUENT

MBS238293-100mL 100mL
EUR 235

ELISA NEPTUNE ASSAY DILUENT

MBS238293-5x100mL 5x100mL
EUR 885

ELISA NEPTUNE ASSAY DILUENT

MBS239230-500mL 500mL
EUR 525

ELISA NEPTUNE ASSAY DILUENT

MBS239230-5x500mL 5x500mL
EUR 2185

ELISA NEPTUNE ASSAY DILUENT

MBS239231-1L 1L
EUR 720

ELISA NEPTUNE ASSAY DILUENT

MBS239231-5x1L 5x1L
EUR 3125

IgM-Reducing Assay Diluent

623 100 mL
EUR 57.25

IgM-Reducing Assay Diluent

625 1 L
EUR 306

Antigen-Down Assay Diluent

630 500 mL
EUR 244

HISPEC ASSAY DILUENT Reagent

GWB-Q00230 50 ml Ask for price

HISPEC ASSAY DILUENT Reagent

GWB-Q00231 125 ml Ask for price

HISPEC ASSAY DILUENT Reagent

GWB-Q00232 500 ml Ask for price

IgM-Reducing Assay Diluent

MBS258386-100mL 100mL
EUR 160

IgM-Reducing Assay Diluent

MBS258386-1L 1L
EUR 355

IgM-Reducing Assay Diluent

MBS258386-500mL 500mL
EUR 305

IgM-Reducing Assay Diluent

MBS258386-5x1L 5x1L
EUR 1485

Antigen-Down Assay Diluent

MBS258393-100mL 100mL
EUR 160

Antigen-Down Assay Diluent

MBS258393-1L 1L
EUR 425

Antigen-Down Assay Diluent

MBS258393-500mL 500mL
EUR 310

Antigen-Down Assay Diluent

MBS258393-5x1L 5x1L
EUR 1780

IgM Reducing Assay Diluent-AD2

KF17348 500 ml
EUR 292.8

ELISA IgM REDUCING ASSAY DILUENT

MBS238292-100mL 100mL
EUR 235

ELISA IgM REDUCING ASSAY DILUENT

MBS238292-5x100mL 5x100mL
EUR 885

ELISA IgM REDUCING ASSAY DILUENT

MBS239228-500mL 500mL
EUR 535

ELISA IgM REDUCING ASSAY DILUENT

MBS239228-5x500mL 5x500mL
EUR 2240

Antigen-Down Assay Diluent-AD4

KF17350 500 ml
EUR 338.4

ELISA Sample Diluent/Assay Buffer

DPGDAB-100mL 100 mL
EUR 59.28

ELISA Sample Diluent/Assay Buffer

DPGDAB-1Liter 1 Liter
EUR 148.2

OOMA00071-25ML - ELISA IgM Assay Diluent

OOMA00071-25ML 25ML
EUR 349

ELISA Sample Diluent/Assay Buffer 10X

DPGDAB-10X-100mL 100 mL
EUR 159.6

ELISA Sample Diluent/Assay Buffer 10X

DPGDAB-10X-1Liter 1 Liter
EUR 1223.6

Assay Diluent Optimization Pack: all four 100mL ADs

MBS258063-1Pack 1Pack
EUR 265

Assay Diluent Optimization Pack: all four 100mL ADs

MBS258063-5Packs 5Packs
EUR 1030

OOSA09576-50ML - HISPEC ASSAY DILUENT Accessory Reagent

OOSA09576-50ML 50ml
EUR 189

OOSA09577-125ML - HISPEC ASSAY DILUENT Accessory Reagent

OOSA09577-125ML 125ml
EUR 409

OOSA09578-500ML - HISPEC ASSAY DILUENT Accessory Reagent

OOSA09578-500ML 500ml
EUR 1479

Assay Diluent Optimization Pack: 100mL AD1, AD2, AD3, AD4

958 pack
EUR 194.5

All Assay Diluents Sample Pack

KF17351 4X100 ml
EUR 326.4

Whole egg yolk (IgY) sample/Conjugate Diluent (Green) for ELISA (antibody assay) concentrate (10X)

80071 50 ml
EUR 242.4

Assay Diluent (Buffered saline with protein stabilisers, surfactant and 0.1 g/L Thiomersal preservative) (120 ml)

R1-02 120 ml
EUR 34
Description: Assay Diluent (Buffered saline with protein stabilisers, surfactant and 0.1 g/L Thiomersal preservative) (120 ml)

Assay Diluent (Buffered saline with protein stabilisers, surfactant and 0.1 g/L Thiomersal preservative) (500 ml)

R1-04 500 ml
EUR 101
Description: Assay Diluent (Buffered saline with protein stabilisers, surfactant and 0.1 g/L Thiomersal preservative) (500 ml)

Diluent A

abx098961-6ml 6 ml
EUR 109.2

Diluent B

abx098962-12ml 12 ml
EUR 109.2

HRP Diluent

85-H10S 250 ml
EUR 266
Description: Dilution and stabilization buffer for monoclonal or polyclonal antibody HRP conjugates

EIA Diluent

2001-100mL 100 mL
EUR 230
Description: Reagents

EIA Diluent

2001-100ml 100 ml
EUR 338.4

EIA Diluent

2001-1L 1 Liter
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IgM Diluent

abx090800-100l 100 µl
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IgM Diluent

abx090800-1ml 1 ml Ask for price

IgM Diluent

abx090800-200l 200 µl Ask for price

ELISA diluent

3652-D2 2 x 120 ml
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Sample Diluent

E-ELIR-011-24Tests 24 Tests Ask for price

Sample Diluent

E-ELIR-011-48Tests 48 Tests
EUR 100

Sample Diluent

E-ELIR-011-96Tests 96 Tests
EUR 20

Sample Diluent

E-ELIR-011-96Tests10 96 Tests *10
EUR 200

Sample Diluent

E-ELIR-011-96Tests5 96 Tests *5
EUR 100

Sample Diluent

I094 1000 ml
EUR 622.8
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent

I094-100 100 ml
EUR 253.2
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent

I094-500 500 ml
EUR 447.6
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent

MBS412097-30mL 30mL
EUR 100

Sample Diluent

MBS412097-5x30mL 5x30mL
EUR 305

Sample Diluent

MBS2571974-100mL 100mL
EUR 95

Sample Diluent

MBS2571974-1L 1L
EUR 160

Sample Diluent, 100ML

X145-100ML 100ML
EUR 112

Antibody Diluent

40125005-1 100 mL
EUR 39.08

Antibody Diluent

40125005-2 500 mL
EUR 90.23

Antibody Diluent

AR1016 100mL (for 10000-15000 assays)
EUR 157.2

Standard Diluent

MBS2090462-1Bottle 1Bottle
EUR 120

Conjugate Diluent

I095 1 L
EUR 678
Description: Conjugate Diluent by Cygnus Technologies is available in Europe via Gentaur.

NHE-BSA Diluent

1Z0216-100mL 100 mL
EUR 176
Description: Reagents

NHE-BSA Diluent

1Z0216-100ml 100 ml
EUR 277.2

NHE-BSA Diluent

1Z0216-1L 1 Liter
EUR 1147.2

Diluent Buffer A

abx296005-10ml 10 ml
EUR 109.2

NADPH Diluent, 5ML

X034-5ML 5ML
EUR 31

Sample Diluent 2.0

MBS689735-15mL 15mL
EUR 125

A549 Sample Diluent, 100mL

F233A-100 100 ml
EUR 308.4
Description: A549 Sample Diluent, 100mL by Cygnus Technologies is available in Europe via Gentaur.

A549 Sample Diluent, 500mL

F233A-500 500 ml
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DNA Sample Diluent

D006-100 100 ml
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DNA Sample Diluent

D006-1000 1000 ml
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DNA Sample Diluent

D006-500 500 ml
EUR 871.2
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Sucrose Diluent 40%

M1749-500G 1 unit
EUR 43.52
Description: Sucrose Diluent 40%

ABC diluent buffer

AR1106-3 12ml
EUR 150
Description: IHC

BSA Sample Diluent

F031A 1000 ml
EUR 585.6
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

BSA Sample Diluent

F031A-100 100 ml
EUR 234
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

BSA Sample Diluent

F031A-500 500 ml
EUR 493.2
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

IgM Sample Diluent

MD-4 each
EUR 80

ABC diluent buffer

MBS176413-12mL 12mL
EUR 135

ABC diluent buffer

MBS176413-5x12mL 5x12mL
EUR 465

AgriseraTMB Diluent (100 ml)

AS15-Diluent 100 ml
EUR 83

Sample Diluent, 60ML

X028-60ML 60ML
EUR 109

Enzyme Label Diluent

ABI500 500 ml
EUR 41.14

Enzyme Label Diluent

ABI999 1000 ml
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Sample diluent bufer

AR1106-1 30ml
EUR 150
Description: ELISA

ELISA Sample Diluent

ESD125 125 ml
EUR 24.43

ELISA Sample Diluent

ESD500 500 ml
EUR 47.58

ELISA Sample Diluent

ESD999 1000 ml
EUR 86.15

HEPES Sample Diluent

I050 1000 ml
EUR 632.4
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

HEPES Sample Diluent

I050-100 100 ml
EUR 262.8
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

HEPES Sample Diluent

I050-500 500 ml
EUR 400.8
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Antibody Diluent Kit

GR103015 100 mL
EUR 68

Antibody Diluent kit

TBS5012 kit
EUR 68

Serum Diluent Buffer

MBS686209-500mL 500mL
EUR 225

Serum Diluent Buffer

MBS686209-5x500mL 5x500mL
EUR 870

TMB Substrate Diluent

42R-TB105 1 liter
EUR 327
Description: Dilution buffer for TMB Substrate in immunoassays

Sample Diluent Buffer

abx296001-20ml 20 ml
EUR 109.2

Conjugate Diluent, 3ML

X076-3ML 3ML
EUR 25

Sample Diluent Buffer

I028-100 100 ml
EUR 253.2
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Sample Diluent Buffer

I028-1000 1000 ml
EUR 622.8
Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent Buffer

I028-500 500 ml
EUR 447.6
Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur.

Conjugate Diluent/HRP

I056 1000 ml
EUR 678
Description: Conjugate Diluent/HRP by Cygnus Technologies is available in Europe via Gentaur.

Sample diluent buffer

MBS176420-30mL 30mL
EUR 135

Sample diluent buffer

MBS176420-5x30mL 5x30mL
EUR 465

TMB Substrate Diluent

MBS538893-1L 1L
EUR 550

TMB Substrate Diluent

MBS538893-5x1L 5x1L
EUR 2365

Lipoprotein (a) Diluent

85-LD01 1 G Ask for price
Description: Lipoprotein (a) deficient serum for use as a diluent in lipoprotein (a) studies

*Lecithin Diluent Broth

M1319-500G 1 unit
EUR 48.17
Description: *Lecithin Diluent Broth

Conjugate Diluent, 13ML

X076-13ML 13ML
EUR 59

Substrate Diluent , 12ML

X101-12ML 12ML
EUR 91

Neptune Sample Diluent

MBS258380-100mL 100mL
EUR 160

Neptune Sample Diluent

MBS258380-1L 1L
EUR 350

Neptune Sample Diluent

MBS258380-500mL 500mL
EUR 295

Neptune Sample Diluent

MBS258380-5x1L 5x1L
EUR 1450

Diluent TSB + Cap 4

LQ504C-10X90ML 1 unit
EUR 41.18
Description: Diluent TSB + Cap 4

Diluent TSB + Cap 4

LQ504CC-5X190ML 1 unit
EUR 44.29
Description: Diluent TSB + Cap 4

Diluent TSB + Cap 4

LQ504D-5X490ML 1 unit
EUR 63.15
Description: Diluent TSB + Cap 4

Standard Diluent Buffer

abx098951-20ml 20 ml
EUR 109.2

HRP-conjugate Diluent

E-ELIR-008-24Tests 24 Tests Ask for price
Family acids (lemon juice and vinegar) improved the strip’s capacity to detect lead by dissolving a number of the lead particulates to the purpose soluble lead exceeded 15 μg/L. These outcomes illustrate the purposes of at-home testing kits for ingesting water evaluation, spotlight limitations and areas for attainable enchancment, and put forth a testing protocol by which new at-home lead check kits may be judged.

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